Vol. 6, Issue 2, Part A (2024)

Synthetic compounds have been used as antimicrobial, anticancer, anti-inflammatory & anti antioxidant from long back. Majority of synthetic drugs like molecules causes the side effects along with their biological activity. In this context, the present study aimed to evaluate the anticytotoxic and anti-haemolytic activity of novel piperazine compound which were proved to be effective antimicrobial agents in the previous studies. Three novel piperazine molecules RL-308, RL-318 & RL-327 were used to evaluate their anticytotoxic effect on non-cancerous Human Foreskin Fibroblasts (HFF) cell lines. Interestingly they could able to exhibit that they are nontoxic up to 1.5 µg/ml. But they exhibit the low toxicity at 2 µg/ml concentration and the same level was continued up to 3 µg/ml. Among three, one piperazine derivative RL-308 was very less toxic. Furthermore, the hemolytic potential was determined against human erythrocytes, which showed that the all the three piperazine derivatives proved to be safe at 1.5 ug concentration when compared to the known hemolytic agent H2O2. Hence, we can conclude that the novel piperazine derivatives are non-toxic to normal human cells and don’t cause lysis in human RBC cells in microgram concentrations.