Vol. 7, Issue 1, Part B (2025)

Pleurotus ostreatus, one of the most cultivated edible mushrooms worldwide, is recognized for its high nutritional value, rapid growth rate, and ability to utilize diverse lignocellulosic substrates. However, achieving a consistently high yield and enriched biochemical profile remains a challenge, especially in regions where substrate quality varies significantly. Biochar an organic, carbon-rich material produced through pyrolysis of biomass has recently gained attention in horticultural and agro-biotechnological applications due to its ability to enhance aeration, water-holding capacity, cation-exchange potential, and microbial colonization in substrates. Studies conducted before 2024 have suggested that biochar amendments can stimulate fungal mycelial growth, improve enzymatic activity, and enhance fruiting body quality in various mushroom species, though research on Pleurotus ostreatus remains limited. This research evaluates the effect of biochar supplementation on the biochemical composition and growth performance of P. ostreatus, focusing particularly on parameters such as mycelial colonization rate, biological efficiency, total phenolics, protein content, ash value, and moisture percentage. By comparing varying levels of biochar integrated into wheat straw-based substrates, the research provides insights into the potential of biochar as a sustainable amendment for improving mushroom productivity while contributing to waste management solutions. The findings support the notion that optimized biochar dosage could significantly enhance the nutraceutical properties of P. ostreatus, making the research relevant for commercial mushroom producers, researchers, and environmental sustainability advocates.