Vol. 7, Issue 2, Part B (2025)

This study examined FTIR analysis of a biosurfactant made by Bacillus subtilis, by using wastewater samples contaminated with organic substances collected from the study area near the Al Ahdeb oil field is situated south of the Al Ahrar District in the Wasit Governorate/ Iraq. These water samples used for isolation of Bacillus subtilis and this bacterium identified according to biochemical tests such as starch hydrolysis, gelatin liquefaction, H2S production, catalase test and acid and gas production. The results of biochemical tests confirmed that the bacterial species belongs to Bacillus subtilis.
Bacillus subtilis used for production of biosurfactant when used in decomposing of organic substances in water samples. FTIR spectroscopy of biosurfactant done by (400-4000) wavenumbers and confirmed that the biosurfactant contains a lipopeptide structure, protein residues and alkyl groups and including different chemical compounds appeared at various wavelengths.